Hemoglobin parameters from diffuse reflectance data

Judith R. Mourant; Oana C. Marina; Tiffany M. Hebert; Gurpreet Kaur; Harriet O. Smith

doi:10.1117/1.JBO.19.3.037004

26 March 2014 Hemoglobin parameters from diffuse reflectance data

Judith R. Mourant, Oana C. Marina, Tiffany M. Hebert, Gurpreet Kaur, Harriet O. Smith

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Journal of Biomedical Optics, Vol. 19, Issue 3, 037004 (March 2014). https://doi.org/10.1117/1.JBO.19.3.037004

Abstract

Tissue vasculature is altered when cancer develops. Consequently, noninvasive methods of monitoring blood vessel size, density, and oxygenation would be valuable. Simple spectroscopy employing fiber optic probes to measure backscattering can potentially determine hemoglobin parameters. However, heterogeneity of blood distribution, the dependence of the tissue-volume-sampled on scattering and absorption, and the potential compression of tissue all hinder the accurate determination of hemoglobin parameters. We address each of these issues. A simple derivation of a correction factor for the absorption coefficient, μ _a , is presented. This correction factor depends not only on the vessel size, as others have shown, but also on the density of blood vessels. Monte Carlo simulations were used to determine the dependence of an effective pathlength of light through tissue which is parameterized as a ninth-order polynomial function of μ _a . The hemoglobin bands of backscattering spectra of cervical tissue are fit using these expressions to obtain effective blood vessel size and density, tissue hemoglobin concentration, and oxygenation. Hemoglobin concentration and vessel density were found to depend on the pressure applied during in vivo acquisition of the spectra. It is also shown that determined vessel size depends on the blood hemoglobin concentration used.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Judith R. Mourant, Oana C. Marina, Tiffany M. Hebert, Gurpreet Kaur, and Harriet O. Smith "Hemoglobin parameters from diffuse reflectance data," Journal of Biomedical Optics 19(3), 037004 (26 March 2014). https://doi.org/10.1117/1.JBO.19.3.037004

Published: 26 March 2014

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