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20 June 2014 Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis
Anthony J. Williams, Jaebum Chung, Xiaoze Ou, Guoan Zheng, Siddarth Rawal, Zheng Ao, Ram Datar, Changhuei Yang, Richard J. Cote M.D.
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Journal of Biomedical Optics, Vol. 19, Issue 6, 066007 (June 2014). https://doi.org/10.1117/1.JBO.19.6.066007
Abstract
Circulating tumor cells (CTCs) are recognized as a candidate biomarker with strong prognostic and predictive potential in metastatic disease. Filtration-based enrichment technologies have been used for CTC characterization, and our group has previously developed a membrane microfilter device that demonstrates efficacy in model systems and clinical blood samples. However, uneven filtration surfaces make the use of standard microscopic techniques a difficult task, limiting the performance of automated imaging using commercially available technologies. Here, we report the use of Fourier ptychographic microscopy (FPM) to tackle this challenge. Employing this method, we were able to obtain high-resolution color images, including amplitude and phase, of the microfilter samples over large areas. FPM’s ability to perform digital refocusing on complex images is particularly useful in this setting as, in contrast to other imaging platforms, we can focus samples on multiple focal planes within the same frame despite surface unevenness. In model systems, FPM demonstrates high image quality, efficiency, and consistency in detection of tumor cells when comparing corresponding microfilter samples to standard microscopy with high correlation (R2=0.99932). Based on these results, we believe that FPM will have important implications for improved, high throughput, filtration-based CTC analysis, and, more generally, image analysis of uneven surfaces.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Anthony J. Williams, Jaebum Chung, Xiaoze Ou, Guoan Zheng, Siddarth Rawal, Zheng Ao, Ram Datar, Changhuei Yang, and Richard J. Cote M.D. "Fourier ptychographic microscopy for filtration-based circulating tumor cell enumeration and analysis," Journal of Biomedical Optics 19(6), 066007 (20 June 2014). https://doi.org/10.1117/1.JBO.19.6.066007
Published: 20 June 2014
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Cited by 81 scholarly publications and 25 patents.
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KEYWORDS
Tumors
Microscopy
Blood
Microscopes
Systems modeling
Imaging systems
Light emitting diodes
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