Biological imaging with coherent Raman scattering microscopy: a tutorial

Alba Alfonso-Garcia; Richa Mittal; Eun Seong Lee; Eric O. Potma

doi:10.1117/1.JBO.19.7.071407

10 March 2014 Biological imaging with coherent Raman scattering microscopy: a tutorial

Alba Alfonso-Garcia, Richa Mittal, Eun Seong Lee, Eric O. Potma

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Journal of Biomedical Optics, Vol. 19, Issue 7, 071407 (March 2014). https://doi.org/10.1117/1.JBO.19.7.071407

Abstract

Coherent Raman scattering (CRS) microscopy is gaining acceptance as a valuable addition to the imaging toolset of biological researchers. Optimal use of this label-free imaging technique benefits from a basic understanding of the physical principles and technical merits of the CRS microscope. This tutorial offers qualitative explanations of the principles behind CRS microscopy and provides information about the applicability of this nonlinear optical imaging approach for biological research.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Alba Alfonso-Garcia, Richa Mittal, Eun Seong Lee, and Eric O. Potma "Biological imaging with coherent Raman scattering microscopy: a tutorial," Journal of Biomedical Optics 19(7), 071407 (10 March 2014). https://doi.org/10.1117/1.JBO.19.7.071407

Published: 10 March 2014

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