Microvascular quantification based on contour-scanning photoacoustic microscopy

Chenghung Yeh; Brian T. Soetikno; Song Hu; Konstantin I. Maslov; Lihong V. Wang

doi:10.1117/1.JBO.19.9.096011

15 September 2014 Microvascular quantification based on contour-scanning photoacoustic microscopy

Chenghung Yeh, Brian T. Soetikno, Song Hu, Konstantin I. Maslov, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 19, Issue 9, 096011 (September 2014). https://doi.org/10.1117/1.JBO.19.9.096011

Abstract

Accurate quantification of microvasculature remains of interest in fundamental pathophysiological studies and clinical trials. Current photoacoustic microscopy can noninvasively quantify properties of the microvasculature, including vessel density and diameter, with a high spatial resolution. However, the depth range of focus (i.e., focal zone) of optical-resolution photoacoustic microscopy (OR-PAM) is often insufficient to encompass the depth variations of features of interest—such as blood vessels—due to uneven tissue surfaces. Thus, time-consuming image acquisitions at multiple different focal planes are required to maintain the region of interest in the focal zone. We have developed continuous three-dimensional motorized contour-scanning OR-PAM, which enables real-time adjustment of the focal plane to track the vessels’ profile. We have experimentally demonstrated that contour scanning improves the signal-to-noise ratio of conventional OR-PAM by as much as 41% and shortens the image acquisition time by 3.2 times. Moreover, contour-scanning OR-PAM more accurately quantifies vessel density and diameter, and has been applied to studying tumors with uneven surfaces.

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Chenghung Yeh, Brian T. Soetikno, Song Hu, Konstantin I. Maslov, and Lihong V. Wang "Microvascular quantification based on contour-scanning photoacoustic microscopy," Journal of Biomedical Optics 19(9), 096011 (15 September 2014). https://doi.org/10.1117/1.JBO.19.9.096011

Published: 15 September 2014

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