Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin

Mihaela Balu; Ilyas Saytashev; Jue Hou; Marcos Dantus; Bruce J. Tromberg

doi:10.1117/1.JBO.20.12.120501

7 December 2015 Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin

Mihaela Balu, Ilyas Saytashev, Jue Hou, Marcos Dantus, Bruce J. Tromberg

Author Affiliations +

Funded by: National Institutes of Health (NIH) NIBIB Laser Microbeam and Medical Program, Air Force Research Laboratory Agreement

Journal of Biomedical Optics, Vol. 20, Issue 12, 120501 (December 2015). https://doi.org/10.1117/1.JBO.20.12.120501

Abstract

Advancing the practical utility of nonlinear optical microscopy requires continued improvement in imaging depth and contrast. We evaluated second‐harmonic generation (SHG) and third‐harmonic generation images from ex vivo human skin and showed that a sub‐40 fs, 1060‐nm Yb‐fiber laser can enhance SHG penetration depth by up to 80% compared to a <100 fs, 800 nm Ti:sapphire source. These results demonstrate the potential of fiber‐based laser systems to address a key performance limitation related to nonlinear optical microscopy (NLOM) technology while providing a low‐barrier‐to‐access alternative to Ti:sapphire sources that could help accelerate the movement of NLOM into clinical practice.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Mihaela Balu, Ilyas Saytashev, Jue Hou, Marcos Dantus, and Bruce J. Tromberg "Sub‐40 fs, 1060‐nm Yb‐fiber laser enhances penetration depth in nonlinear optical microscopy of human skin," Journal of Biomedical Optics 20(12), 120501 (7 December 2015). https://doi.org/10.1117/1.JBO.20.12.120501

Published: 7 December 2015

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Cited by 21 scholarly publications.

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KEYWORDS

Skin

Second-harmonic generation

Microscopy

Optical microscopy

Fiber lasers

Collagen

Laser scattering

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