Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy

Surekha Barkur; Aseefhali Bankapur; Madhura Pradhan; Santhosh Chidangil; Deepak Mathur; Uma Ladiwala

doi:10.1117/1.JBO.20.8.085001

5 August 2015 Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy

Surekha Barkur, Aseefhali Bankapur, Madhura Pradhan, Santhosh Chidangil, Deepak Mathur, Uma Ladiwala

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Journal of Biomedical Optics, Vol. 20, Issue 8, 085001 (August 2015). https://doi.org/10.1117/1.JBO.20.8.085001

Abstract

Single-cell micro-Raman spectroscopy has been applied to explore cell differentiation in single, live, and malignant cells from two tumor cell lines. The spectra of differentiated cells exhibit substantial enhancement primarily in the intensities of protein peaks with concomitant decrease in intensities of O─P─O asymmetric stretching peaks in DNA/RNA. Principal component analyses show that the spectral score of differentiated cells tends to asymptotically approach that of spectra obtained from normal neural stem cells/progenitors. This lends credence to the notion that the observed spectral changes are specific to differentiation, since upon differentiation, malignant cells become less malignant and tend toward benignity.

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Surekha Barkur, Aseefhali Bankapur, Madhura Pradhan, Santhosh Chidangil, Deepak Mathur, and Uma Ladiwala "Probing differentiation in cancer cell lines by single-cell micro-Raman spectroscopy," Journal of Biomedical Optics 20(8), 085001 (5 August 2015). https://doi.org/10.1117/1.JBO.20.8.085001

Published: 5 August 2015

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