Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy

Chukwuemeka Okoro; Kimani C. Toussaint

doi:10.1117/1.JBO.21.1.016011

26 January 2016 Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy

Chukwuemeka Okoro, Kimani C. Toussaint

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Journal of Biomedical Optics, Vol. 21, Issue 1, 016011 (January 2016). https://doi.org/10.1117/1.JBO.21.1.016011

Abstract

We present the first experimental demonstration of two-photon Mueller matrix second-harmonic generation (SHG) microscopy for the extraction of representative Mueller matrices. Individual SHG images of porcine sample sets are divided into subimages, and the local nonlinear two-photon Mueller matrices (M⁽²⁾) and degree of polarization (p) are determined. Results of the two-photon Mueller matrices from different sample types and the effects of thickness on the mean p distribution per polarization basis are investigated. We find that the p distribution shape changes and an associated bimodal mean difference (μ_d) increases with thickness. Our approach has potential as a quantitative imaging technique and can be applied to other multiphoton coherent imaging modalities.

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Chukwuemeka Okoro and Kimani C. Toussaint "Experimental demonstration of two-photon Mueller matrix second-harmonic generation microscopy," Journal of Biomedical Optics 21(1), 016011 (26 January 2016). https://doi.org/10.1117/1.JBO.21.1.016011

Published: 26 January 2016

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Cited by 15 scholarly publications and 1 patent.

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KEYWORDS

Second-harmonic generation

Polarization

Microscopy

Tissues

Mueller matrices

Collagen

Multiphoton microscopy

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