Polarization response of second-harmonic images for different collagen spatial distributions

Francisco J. Ávila; Oscar del Barco; Juan M. Bueno

doi:10.1117/1.JBO.21.6.066015

22 June 2016 Polarization response of second-harmonic images for different collagen spatial distributions

Francisco J. Ávila, Oscar del Barco, Juan M. Bueno

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Journal of Biomedical Optics, Vol. 21, Issue 6, 066015 (June 2016). https://doi.org/10.1117/1.JBO.21.6.066015

Abstract

The response to polarization of second-harmonic generation (SHG) microscopy images of samples with different collagen distributions (quasialigned, partially organized, and nonorganized) has been analyzed. A linear decay relationship between the external arrangement and polarization sensitivity was found. SHG signal from nonorganized samples presented a large structural dispersion and a weak dependence with incident polarization. Polarization dependence is also associated with the internal organization of the collagen fibers, directly related to the ratio of hyperpolarizabilities ρ. This parameter can experimentally be computed from the modulation of the SHG signal. The results show that both external and internal collagen structures are closely related. This provides a tool to obtain information of internal properties from the polarimetric response of the external spatial distribution of collagen, which might be useful in clinical diagnosis of pathologies related to changes in collagen structure.

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Francisco J. Ávila, Oscar del Barco, and Juan M. Bueno "Polarization response of second-harmonic images for different collagen spatial distributions," Journal of Biomedical Optics 21(6), 066015 (22 June 2016). https://doi.org/10.1117/1.JBO.21.6.066015

Published: 22 June 2016

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