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13 July 2016 Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy
Thiago de Oliveira Mendes, Liliane P. Pereira, Laurita dos Santos, Vamshi K. Tippavajhala, Claudio Téllez Soto, Airton Abrahão Martin
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Journal of Biomedical Optics, Vol. 21, Issue 7, 075010 (July 2016). https://doi.org/10.1117/1.JBO.21.7.075010
Abstract
The analysis of biological systems by spectroscopic techniques involves the evaluation of hundreds to thousands of variables. Hence, different statistical approaches are used to elucidate regions that discriminate classes of samples and to propose new vibrational markers for explaining various phenomena like disease monitoring, mechanisms of action of drugs, food, and so on. However, the technical statistics are not always widely discussed in applied sciences. In this context, this work presents a detailed discussion including the various steps necessary for proper statistical analysis. It includes univariate parametric and nonparametric tests, as well as multivariate unsupervised and supervised approaches. The main objective of this study is to promote proper understanding of the application of various statistical tools in these spectroscopic methods used for the analysis of biological samples. The discussion of these methods is performed on a set of in vivo confocal Raman spectra of human skin analysis that aims to identify skin aging markers. In the Appendix, a complete routine of data analysis is executed in a free software that can be used by the scientific community involved in these studies.
© 2016 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2016/$25.00 © 2016 SPIE
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Thiago de Oliveira Mendes, Liliane P. Pereira, Laurita dos Santos, Vamshi K. Tippavajhala, Claudio Téllez Soto, and Airton Abrahão Martin "Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy," Journal of Biomedical Optics 21(7), 075010 (13 July 2016). https://doi.org/10.1117/1.JBO.21.7.075010
Published: 13 July 2016
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Cited by 3 scholarly publications.
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KEYWORDS
Statistical analysis
Skin
Raman spectroscopy
Confocal microscopy
In vivo imaging
Biological research
Principal component analysis
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