Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

Chenxi Li; Ruikang K. Wang

doi:10.1117/1.JBO.22.4.046002

6 April 2017 Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal

Chenxi Li, Ruikang K. Wang

Author Affiliations +

Journal of Biomedical Optics, Vol. 22, Issue 4, 046002 (April 2017). https://doi.org/10.1117/1.JBO.22.4.046002

Abstract

We propose an approach to measure heterogeneous velocities of red blood cells (RBCs) in capillary vessels using full-field time-varying dynamic speckle signals. The approach utilizes a low coherent laser speckle imaging system to record the instantaneous speckle pattern, followed by an eigen-decomposition-based filtering algorithm to extract dynamic speckle signal due to the moving RBCs. The velocity of heterogeneous RBC flows is determined by cross-correlating the temporal dynamic speckle signals obtained at adjacent locations. We verify the approach by imaging mouse pinna in vivo, demonstrating its capability for full-field RBC flow mapping and quantifying flow pattern with high resolution. It is expected to investigate the dynamic action of RBCs flow in capillaries under physiological changes.

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Chenxi Li and Ruikang K. Wang "Velocity measurements of heterogeneous RBC flow in capillary vessels using dynamic laser speckle signal," Journal of Biomedical Optics 22(4), 046002 (6 April 2017). https://doi.org/10.1117/1.JBO.22.4.046002

Received: 14 February 2017; Accepted: 21 March 2017; Published: 6 April 2017

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