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17 October 2018 Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy
Janel L. Davis, Biqin Dong, Cheng Sun, Hao F. Zhang
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Journal of Biomedical Optics, Vol. 23, Issue 10, 106501 (October 2018). https://doi.org/10.1117/1.JBO.23.10.106501
Abstract
The existence of fluorescent impurities has been a long-standing obstacle in single-molecule imaging, which results in sample misidentification and higher localization uncertainty. Spectroscopic single-molecule localization microscopy can record the full fluorescent spectrum of every stochastic single-molecule emission event. This capability allows us to quantify the spatial and spectral characteristics of fluorescent impurities introduced by sample preparation steps, based on which we developed a method to effectively separate fluorescent impurities from target molecules.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Janel L. Davis, Biqin Dong, Cheng Sun, and Hao F. Zhang "Method to identify and minimize artifacts induced by fluorescent impurities in single-molecule localization microscopy," Journal of Biomedical Optics 23(10), 106501 (17 October 2018). https://doi.org/10.1117/1.JBO.23.10.106501
Received: 9 July 2018; Accepted: 21 September 2018; Published: 17 October 2018
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Cited by 13 scholarly publications.
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KEYWORDS
Molecules
Plasma
Laser phosphor displays
Microscopy
Optical filters
Luminescence
Stochastic processes
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