Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsun-Chia Hsu; Lei Li; Junjie Yao; Terence T.W. Wong; Junhui Shi; Ruimin Chen; Qifa Zhou; Lihong V. Wang

doi:10.1117/1.JBO.23.7.076001

6 July 2018 Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy

Hsun-Chia Hsu, Lei Li, Junjie Yao, Terence T.W. Wong, Junhui Shi, Ruimin Chen, Qifa Zhou, Lihong V. Wang

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Journal of Biomedical Optics, Vol. 23, Issue 7, 076001 (July 2018). https://doi.org/10.1117/1.JBO.23.7.076001

Abstract

Optical-resolution photoacoustic microscopy (OR-PAM) has demonstrated fast, label-free volumetric imaging of optical-absorption contrast within the quasiballistic regime of photon scattering. However, the limited numerical aperture of the ultrasonic transducer restricts the detectability of the photoacoustic waves, thus resulting in incomplete reconstructed features. To tackle the limited-view problem, we added an oblique illumination beam to the original coaxial optical-acoustic scheme to provide a complementary detection view. The virtual augmentation of the detection view was validated through numerical simulations and tissue-phantom experiments. More importantly, the combination of top and oblique illumination successfully imaged a mouse brain in vivo down to 1 mm in depth, showing detailed brain vasculature. Of special note, it clearly revealed the diving vessels that were long missing in images from original OR-PAM.

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Hsun-Chia Hsu, Lei Li, Junjie Yao, Terence T.W. Wong, Junhui Shi, Ruimin Chen, Qifa Zhou, and Lihong V. Wang "Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy," Journal of Biomedical Optics 23(7), 076001 (6 July 2018). https://doi.org/10.1117/1.JBO.23.7.076001

Received: 9 March 2018; Accepted: 8 June 2018; Published: 6 July 2018

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