Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope

Chengbo Yin; Linpeng Wei; Sanjee Abeytunge; Gary Peterson; Milind Rajadhyaksha; Jonathan T. C. Liu

doi:10.1117/1.JBO.24.3.030501

27 March 2019 Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope

Chengbo Yin, Linpeng Wei, Sanjee Abeytunge, Gary Peterson, Milind Rajadhyaksha, Jonathan T. C. Liu

Author Affiliations +

Journal of Biomedical Optics, Vol. 24, Issue 3, 030501 (March 2019). https://doi.org/10.1117/1.JBO.24.3.030501

Abstract

There would be clinical value in a miniature optical-sectioning microscope to enable in vivo interrogation of tissues as a real-time and noninvasive alternative to gold-standard histopathology for early disease detection and surgical guidance. To address this need, a reflectance-based handheld line-scanned dual-axis confocal microscope was developed and fully packaged for label-free imaging of human skin and oral mucosa. This device can collect images at >15 frames / s with an optical-sectioning thickness and lateral resolution of 1.7 and 1.1 μm, respectively. Incorporation of a sterile lens cap design enables pressure-sensitive adjustment of the imaging depth by the user during clinical use. In vivo human images and videos are obtained to demonstrate the capabilities of this high-speed optical-sectioning microscopy device.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Chengbo Yin, Linpeng Wei, Sanjee Abeytunge, Gary Peterson, Milind Rajadhyaksha, and Jonathan T. C. Liu "Label-free in vivo pathology of human epithelia with a high-speed handheld dual-axis confocal microscope," Journal of Biomedical Optics 24(3), 030501 (27 March 2019). https://doi.org/10.1117/1.JBO.24.3.030501

Received: 21 November 2018; Accepted: 13 March 2019; Published: 27 March 2019

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KEYWORDS

Microscopes

Confocal microscopy

Tissues

Mirrors

Tissue optics

In vivo imaging

Microelectromechanical systems

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