CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 5 > Issue 2 > Article
Open Access
1 April 2000 Feasibility of field-based light scattering spectroscopy
Changhuei Yang, Lev T. Perelman, Adam Wax, Ramachandra R. Dasari, Michael S. Feld
Author Affiliations +
Journal of Biomedical Optics, Vol. 5, Issue 2, (April 2000). https://doi.org/10.1117/1.429980
Light scattering spectroscopy (LSS) is a new technique capable of accurately measuring the features of nuclei and other cellular organelles in situ. We present the considerations required to implement and interpret field-based detection in LSS, where the scattered electric field is detected interferometrically, and demonstrate that the technique is experimentally feasible. A theoretical formalism for modeling field-based LSS signals based on Mie scattering is presented. Phase-front uniformity is shown to play an important and novel role. Results of heterodyne experiments with polystyrene microspheres that localize LSS signals to a region about 30 µm in axial extent are reported. In addition, differences between field-based LSS and the earlier intensity-based LSS are discussed.
Citation Download Citation
Changhuei Yang, Lev T. Perelman, Adam Wax, Ramachandra R. Dasari, and Michael S. Feld "Feasibility of field-based light scattering spectroscopy," Journal of Biomedical Optics 5(2), (1 April 2000). https://doi.org/10.1117/1.429980
Published: 1 April 2000
JOURNAL ARTICLE
 6 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 24 scholarly publications.
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Light scattering
Heterodyning
Scattering
Sensors
Laser beam diagnostics
Mie scattering
Spectroscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top