Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods

Enrico Gratton; Sophie Breusegem; Jason D. B. Sutin; Qiaoaio Ruan; Nicholas P. Barry

doi:10.1117/1.1586704

1 July 2003 Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods

Enrico Gratton, Sophie Breusegem, Jason D. B. Sutin, Qiaoaio Ruan, Nicholas P. Barry

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Journal of Biomedical Optics, Vol. 8, Issue 3, (July 2003). https://doi.org/10.1117/1.1586704

Fluorescence lifetime images are obtained with the laser scanning microscope using two methods: the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.

©(2003) Society of Photo-Optical Instrumentation Engineers (SPIE)

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Enrico Gratton, Sophie Breusegem, Jason D. B. Sutin, Qiaoaio Ruan, and Nicholas P. Barry "Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods," Journal of Biomedical Optics 8(3), (1 July 2003). https://doi.org/10.1117/1.1586704

Published: 1 July 2003

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