Lighting gel filters are widely used in commercial industries, but their adoption in scientific applications is limited, despite their low cost and form factor advantages. Here, we compare the optical performance of lighting gel filters to commonly used dielectric and colored glass filters in terms of absorbance spectra, passband transmission, angle of incidence dependence, autofluorescence, and photostability. Further comparison is performed in both preclinical and clinical imaging applications. The results show that gel filters might be a superior filter choice in several optical systems, including compact designs and fluorescence imaging applications. Compact designs using gel filters could have a significant advantage for applications such as point-of-care diagnostics, smartphone device add-ons, and single-use fluorescent assays.