We used diffuse reflectance spectroscopy to assess the effects of different radiation therapy doses on tumor microenvironment. We injected 4T1 cells into the flanks of 25 mice to develop tumor xenografts, and randomly distributed the animals into control and radiation groups, which received a dose of either 1, 2, or 4 Gy. Treatment started when tumors reached a volume of 200 mm3. DRS measurements were obtained prior to and 1 hour after radiation on the five days of treatment, and once a day thereafter. Our data demonstrates that DRS is sensitive to tumor microenvironmental changes after low doses of radiation.
We have used Raman spectroscopy to assess biomolecular changes of two human head and neck cancer xenografts in response to radiation therapy. We injected radiation-sensitive UM-SCC-22B and –resistant UM-SCC-47 cell lines into the flanks of 80 mice to grow tumor xenografts. Animals were distributed into control and radiation groups where the latter group received a single dose of 2 Gy. In vivo Raman spectroscopy was conducted before, 1, 24, and 48 hours after radiation and data were decomposed using principal component analysis and multi variate curve resolution. We found statistically significant differences in biomolecular changes among radiation-sensitive and –resistant groups.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.