Mr. Berhanu K. Wondimu Profile

Berhanu K. Wondimu

at San Diego State Univ

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Proceedings Article | 17 November 2008 Paper

High-efficiency microarray of 3-D carbon MEMS electrodes for pathogen detection systems

Sam Kassegne, Berhanu Wondimu, Mohammad Majzoub, Jiae Shin

Proceedings Volume 7266, 726615 (2008) https://doi.org/10.1117/12.806624

KEYWORDS: Electrodes, Carbon, Pathogens, Microelectromechanical systems, Diagnostics, Particles, Packaging, Silicon, Silicon carbide, Silver

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Molecular diagnostic applications for pathogen detections require the ability to separate pathogens such as bacteria, viruses, etc., from a biological sample of blood or saliva. Over the past several years, conventional two-dimensional active microarrays have been used with success for the manipulation of biomolecules including DNA. However, they have a major drawback of inability to process relatively 'largevolume' samples useful in infectious disease diagnostics applications. This paper presents an active microarray of three-dimensional carbon electrodes that exploits electrokinetic forces for transport, accumulation, and hybridization of charged bio-molecules with an added advantage of large volume capability. Tall 3-dimensional carbon microelectrode posts are fabricated using C-MEMS (Carbon MEMS) technology that is emerging as a very exciting research area since carbon has fascinating physical, chemical, mechanical and electrical properties in addition to its low cost. The chip fabricated using CMEMS technology is packaged and its efficiency of separation and accumulation of charged particle established by manipulating negatively charged polycarboxylate 2 μm beads in 50 mM histidine buffer.

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