Stressful states of the body are accompanied by a hormonal response, including an increase in the level of the hormone cortisol. Due to the fact that cortisol is contained in many biological fluids of the human body, as well as accumulates in some tissues, researchers are attracted to the possibility of determining cortisol by point-of-care methods, which include immunochromatographic analysis. This paper discusses some important practical nuances that need to be taken into account when creating a test strip for detecting cortisol, for example, in human salivary fluid. Keywords: cortisol, gold nanoparticles, stress, saliva, lateral flow immunoassay, test strips. 1. INTRODUCTION
Psycho-emotional stress is the cause of many diseases. Cortisol is a marker of psychoemotional stress, an increase in the level of this hormone in human biological fluids may indicate experienced stress. As a physiological indicator of the level of stress experienced, the content of cortisol in human biological fluids is used. The most convenient detection of cortisol in saliva. Immunochromatography allows you to get the result outside the hospital and in a short period of time. However, one of its significant drawbacks is the qualitative rather than quantitative nature of the analysis. In this work, we propose conducting a dot-immunoassay of cortisol in saliva using a thermographic method and comparing it with visual observation.
Freeze-drying as known as lyophilization has been considered as a possible technique to improve the long-term stability of colloidal luminescent quantum dots (QDs) for perspective biomedical application. The paper describes synthesis of biocompatible CdSe-based core/shell QDs and discusses their optical and physical properties before and after freezedrying. Importantly, the dried nanoparticles can be stored for a long time under usual conditions and then can easily be redisperse in water at a desired concentration without such hard manipulations as sonication or heating. In this work two PEG-amine derivatives were applied for QDs pegylation: monoamine Jeffamine M1000 and diamine JeffamineED-2003. The use of different Jeffamines allows us to obtain QDs with different length of PEG chains and different ζ-potential. The influence of polymer composition on optical properties of the nanocrystals and on their stability after freeze-drying was studied.
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