Otitis media (OM) is a common middle ear disease that is treated with antibiotics. However, over-prescription of antibiotics heightens the risk of antibiotic resistance. Here, we report the development and testing of a new cold microplasma (CMP) device to treat OM, and demonstrate the translation for in vivo use in a chinchilla animal model. In vitro nontypeable Haemophilus influenzae bacterial and biofilm samples and ex vivo tissue specimens were evaluated for inactivation and injury. CMP-induced effects on any infectious symptoms (middle ear fluid, biofilms) were longitudinally observed with OCT. This represents the first application of CMP treatments for OM therapy.
A middle ear infection is a prevalent inflammatory disease during childhood, often caused by bacterial pathogens. A portable and replaceable microplasma jet array was developed to investigate the feasibility of inactivating Pseudomonas aeruginosa, a common bacterial strain associated with middle ear infections. Reactive species generated by the non-thermal microplasma jet array inactivated planktonic bacteria and biofilm. A middle ear phantom was developed using the rat eardrum to study the antimicrobial effects on bacteria located behind the eardrum. Lastly, 3D volumetric OCT imaging and histology were performed on the rat eardrum to examine the potential structural changes due to the plasma.
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