In this paper, a new complex sensing film containing both optical indicator and enzyme was prepared and its sensing
properties were studied, using cellulose acetate (CA) as the carrier and tris (2,2'-blpyridyl) dichloro-ruthenium (II)
hexahydrate (Ru(bpy)3Cl2) as the indicator. The cross-linking method was used to immobilize glucose oxidase (GOD).
The immobilization conditions were optimized: the concentration of sodium periodate as 0.2 M and the reaction time as
30 min, those for ethanediamine as 0.03 M and 2.5 hours, those for GA as 1.5% (v/v) and 2 hours, those for GOD as 35
mg/ml and 21h. The optimal temperature and pH value for the catalytic properties of the sensing film are 38 °C and 7.0,
respectively. A fiber optic glucose sensor with this complex sensing film has been studied. The results show that its
detecting range is 100-600 mg/dl and its response time is less than 20 seconds.
In this paper, Glucose oxidase (GOD) was immobilized on a novel silica membrane. The multifunctional sensing
membrane was prepared by sol-gel method. GOD was immobilized on the aminated silica surfaces by glutaraldehyde
cross-linking method. The fiber optical glucose sensor based on fluorescence quenching was designed and fabricated
using lock-in amplifying technology to realize the detection of glucose concentration. The experimental results show that
a linear range between phase delay φ and the glucose concentration of the solution was observed in the concentration
range of 100 to 600 mg/dl and the detection limit is 50mg/dl, the sensor can meet the demand of clinical application. The
response time of the sensing membrane was about 15s. The experimental results demonstrated that this biosensor with
the multifunctional sensing membranes has high sensitivity, repeatability, good stability and fast response.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.