With the development of microscopic camera, focusing technology, which is an indispensable component of microscopy camera, constantly develops in the field of automation and high precision. The auto focusing microscopy camera which extremely meets the needs of this period appears at the same time. The climbing searching method is one of the most widely used fast search algorithms on microscopy camera. But the method has its shortcomings. It is weak in anti-noise capability and it can’t correctly identify the search direction in the area far from the focus. It will cause the camera fail to focus accurately and quickly. This paper proposes an implementation method of an auto focusing microscopic camera based on the UVC protocol, which can effectively solve the shortcomings of the climbing searching method and achieve fast , accurate focusing. At the same time, based on the UVC protocol, the microscopy camera outputs and is controlled through the USB3.0 interface, which improves the convenience of use.
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