The aim of this study was to evaluate a relatively new calcium silicate cement (Biodentine(R)) used as a dentine substitute. The marginal adaptation in Class II restorations have been studied; this is the spot, where gingival margins are situated apical to the cemento-enamel junction (CEJ). 20 Class II cavities have been prepared on mesial and distal surfaces of 10 extracted teeth, with margins extending 1 mm below the cementum-enamel junction. Cavities have been restored with composite resin and Biodentine(R) in an "open sandwich" technique. The assessment of the marginal adaptation was performed using Laser Scanning Confocal Microscopy. The considered interfaces have been evaluated and the differences between the different materials have been discussed. In conclusion, contemporary calcium silicate materials, such as Biodentine, can be used as substitute materials for the dentin. However, because Biodentine needs extensive time to set, the operating time is longer than when a Resin-Modified Glass-Ionomer (RMGI) is used.
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