The polysaccharide in Pseudostellaria heterophylla (PH) were detected by ultraviolet-visible spectrophotometry combined with different processing methods. Different processing methods had a significant influence on the content of Polysaccharide in PH, especially the difference between evaporated prior to shade-dried products and other three samples. The sample had a large influence on the content of Polysaccharide by evaporating, and the sorting of the sample content after treatment of different processing methods is as follows: evaporated prior to shade-dried products>sun-dried products>oven dried products>shade-dried products. This study provided theoretical and practical basis for the processing and clinical application of PH.
Based on High Performance Liquid Chromatography-Diode Array Detection (HPLC-DAD) and chemical pattern recognition, this study constructed 12 batches of polygonatum sibiricum spectral fingerprinting from different areas and sources. Pattern recognition techniques such as similarity evaluation, Principal Component Analysis (PCA) and Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) were used in the research process to screen out the characteristic components as Quality Markers (Q-Markers) of polygonatum sibiricum. A total of 16 common peaks were found in spectral fingerprinting, with similarity evaluation in the range of 0.686 to 0.918. In the PCA analysis, it was found that the three principal components (PC1, PC2 and PC3) characterized the variation information (35.04%, 25.15% and 14.56%) of the original variables, and the cumulative contribution value reached 74.75%. OPLS-DA analysis was used to screen out 6 characteristic components, which are considered potential Q-Markers of polygonatum sibiricum. In this study, the characteristic components were selected as Q-Markers, which provided a reference for the improvement of the quality standard of polygonatum sibiricum.
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