Antônio L. Pinheiro, Luiz G. Soares, Aline C. da Silva, Nicole R. Santos, Anna Paula L. T. da Silva, Bruno Luiz R. C. Neves, Amanda Soares, Landulfo Silveira
The aim of the present study was to assess, by means of Raman spectroscopy, the repair of complete surgical tibial fractures fixed with wire osteosynthesis or miniplates treated or not with infrared laser (λ780 nm) or infrared LED (λ850 ± 10 nm) lights, 142.8 J/cm2 per treatment, associated or not to the use of mineral trioxide aggregate (MTA) cement. Surgical fractures were created on 36 rabbits and fixed with WO or miniplates and some groups were grafted with MTA. Irradiated groups received lights at every other day for 15 days and sacrifice occurred after 30 days. The results showed that only irradiation with either laser or LED influenced the peaks of phosphate (~960 cm-1) and carbonated (~1,070 cm-1) hydroxyapatite. Collagen peak (1,450 cm-1) was influenced by both the use of MTA and irradiation with either laser or LED. It is concluded that the use of either laser or LED phototherapy associated to MTA cement was efficacious on improving the repair of complete tibial fractures treated with wire osteosynthesis or miniplates.
Beside biomaterials, Laser phototherapy has shown positive effects as auxiliary therapy in bone repair process,
especially when involving large bone losses. The aim of this histological study was to evaluate, by light microscopy, the
influence of laser phototherapy on the repair of complete tibial fractures in rabbits treated or not with semi-rigid internal
fixation and Mineral Trioxide Aggregate - MTA graft. Twelve Rabbits were randomly divided into four groups with
three animals each. After general anesthesia, complete fractures were created in one tibia with a carborundum disk. All
animals (groups I-IV) had the fracture stabilized with semi-rigid fixation (wire osteosynthesis - WO). Group I was
routinely fixed with WO; groups II and IV fracture was filled by blood clot and MTA implant. In Groups III and IV
fracture was filled by blood clot and further irradiated with laser (λ780 nm, 70 mW, CW, Φ = 0.04 cm2, 20.4 J/cm2, per
session, t = 300s, 142.8 J/cm2 per treatment). The phototherapy protocol was applied immediately after the surgery and
repeated each 48 hours during 15 days. Animal death occurred on the 30th postoperative day. After removal of the
specimens, the samples were routinely processed, stained with HE and evaluated by light microscopy. Histologically,
the group treated with MTA graft and irradiated with laser showed the fracture filled by a more organized and mature
trabecular bone, when compared with all other groups. From the results of the present study, it may be concluded that the
association of Laser phototherapy + MTA graft in fractures treated with WO improved bone repair when compared with
fractures treated only with WO.
We aimed assess the effects of different energy densities of the association of red/IR laser light on the healing
of cutaneous wounds infected Staphylococcus aureus. Background: Wound infection is the most common
complication on healing wounds and cause both vascular and cellular responses on the tissue. Several therapeutics is
used for improving wound healing including the use of different light sources, such as the Laser. Some energy
densities present positive photobiological effects on the healing process. Material and Methods: 24 young adult
male Wistar rats, under general anesthesia, had their dorsum shaven, cleaned and a 1 x 1cm cutaneous wound created
with a scalpel and left without no suturing or dressings. The wounds were infected with Staphylococcus aureus and
were randomly divided in 8 subgroups of 3 animals in each: Control, Group 10J/cm2, Group 20J/cm2, and Group
30J/cm2, 7 and 14 days each group. Laser phototherapy was carried out with a diode (λ680nm/790nm, P=
30mW/40mW, CW, Laser, Ø = 3mm, PD=424mW/cm2 and 566mW/cm2, t=11.8/ 8.8 sec, E=0.35J) and started
immediately after surgery and repeated at every other day during 7 days. Laser light was applied on 4 points around
wounded area. The animals were killed at either 8th or 15th day after contamination. Specimens were taken, routinely
cut and processed to wax, stained and underwent histological analysis. The results were statistically analyzed.
Results: Both 20 and 30J/cm2 caused intense collagen deposition at the end of the experimental time. But, when 20
J/cm2 was used the fibers were also well organized. Conclusion: Our results indicate that irradiated subjects showed
improved wound healing being the 20 J/cm2 the energy the caused better histological response.
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