Arnoud Everhardt, Vasileios Konstantinidis, Paul Kapteijn, Marcel Hoekman, Soultana Konstantinidou, Marta D'Amora, Alessandro De Carli, Francesco Fuso, Francesco Tantussi, Michele Lai, Vittoria Raffa, Sadoon Al-Obaidi, Noor Schilder, René Heideman, Douwe Geuzebroek, Frederik Schreuder
Genome editing comprises the most promising work in 21st century genetics, with molecular surgery as its practical horizon. For a successful clinical application of molecular surgery, e.g. to treat cancer, safe and efficient editing is crucial. Light-induced molecular surgery is the perfect tool for its excellent control over wavelength, power, and exposure time. Light is also an excellent tool for the detection of (living) cells. These modalities are ideal for opto-fluidics: combining integrated photonics and microfluidics in a chip. In this work, we used the TriPleX® waveguide platform, comprised of silicon nitride and silicon oxide, to control high power (>1W) visible light. Living cells are inserted into a 100 micrometer wide microfluidic channel after which they are focused into a 25 micrometer wide section in its center using side-, backand lift sheath flows. The cells can be readily recovered at the microfluidic channel’s output with >90% survival rate. Chemically deactivated CRISPR/Cas9 molecules are activated by the laser light for safe molecular surgery. In parallel, it is possible to detect living cells flowing in the microfluidic channel. Measuring light absorption by the analyte makes it possible to detect each individual cell passing by the laser light, and to microscopically verify that >97% of the cells are correctly centered in the microfluidic channel. This device represents a first step to a fully integrated on-chip flow cytometer. Early results demonstrate its efficacy in cell detection and controllable exposure, paving the way to safe molecular surgery.
Currently, in systems using Photonic Integrated Circuits (PIC), not many integrated options for lasers on-chip are available. So generally, off-chip devices (pig-tailed lasers, etc.) or underperforming on-chip devices are used. From both price and performance point of view, this is an undesirable situation. Especially, for automotive-grade solid-state FMCW LiDAR systems, the elephant in the room is generally ignored; the optical output power generated by the laser is too low, and/or the mode-hop free tuning is too little and too slow. In this presentation, we will show designs and expected results that offer a customized laser suitable for FMCW LiDAR, with powers only limited by nonlinear effects in the PIC platform, wavelength tuning ranges of over 100 nm and Lorentzian linewidths appropriate to that of a distance measurement of 300 m (source to target). Our current hybrid integration solution is targeted at c-band, but the approach is valid for all wavelengths for which the PIC platform is transparent.
We have developed a compact PIC external cavity laser consisting of a hybrid integrated InP gain section and SiN tunable mirror, with a superior combination of characteristics. The laser has shown a narrow linewidth < 5 kHz, broad tuning range of 140 nm over the S-, C- and L- band, from 1473 nm to 1612 nm, and high single mode output power of 60 mW. The laser frequency can be modulated at frequencies < 10 MHz having a wavelength modulation depth of < 20 MHz.
We present a novel hybrid 800 nm laser with a wide tuning range, high optical power and ultra-narrow linewidth with ⪆kHz tuning speeds and a small footprint. Tunable, narrow linewidth hybrid lasers around 800nm serve as an attractive choice for e.g. OCT, LIDAR and atomic transition locking in e.g. atomic clocks. The laser has a microring resonator based optical cavity. The laser has a tuning range of 45 nm and a maximum output power of 5 dBm. The intrinsic linewidth of the laser is measured at 22 kHz.
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