Liver transplantation is a life-saving procedure for patients with end-stage liver diseases. However, the unbalance between number of transplanted patients and patients on waiting list is still an issue. The introduction of perfusion machines and the use of marginal organs tend to reduce this unbalance. However, all marginal organs cannot be transplanted as they suffer more frequently from post-transplant complications. Today there is no efficient method to assess the viability of the organ and therefore to select the marginal organs suitable transplantation. Tissue autofluorescence is an optical method which enables to detect metabolic activity and offer insights about liver viability. Our preliminary results with fluorescence measurements show that we can measure the relative concentration of different mitochondria biomarkers such as flavin, NADH and PpIX under different ischemia-reperfusion conditions on porcine model.
Fatty liver disease, or steatosis, is a pathology characterized by the presence of fat droplets in the cytoplasm of hepatocytes. This common and potentially severe condition can result in liver damage and various health complications. The current gold standard method to assess steatosis involves an invasive biopsy and a subjective anatomopathological analysis. In this study, we introduce a novel non-invasive approach based on near infrared diffuse reflectance spectroscopy. Currently, NIR DRS methods are often use to quantify fat fraction but our method provides a way to quantify a fraction of hepatocytes affected by steatosis and thus can be directly compared to anatomopathological analysis. The results obtained from this method demonstrate a strong correlation with the gold standard.
We develop a method to measure liver steatosis percentage through relative quantification of fat with near infrared diffuse reflectance spectroscopy. The results obtained show a good correlation with the gold standard anatomopathological analysis.
We present a method to assess steatosis percentage and relative quantification of fat in the liver using near infrared diffuse reflectance spectroscopy. Measurements performed are in good agreement with the gold standard anatomopathological results.
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