We have developed a new µOCT tethered Capsule Endomicroscopy imaging system for the diagnosis of Inflammatory bowel disease and Gastrointestinal cancer with micrometer resolution. The capsule we built measures 30 mm in length and 11 mm in diameter. By employing an optical design that incorporates the mirror tunnel design, we achieved a lateral resolution of less than 4 µm across a depth of field of 450 µm. The spectrometer we designed provides a resolution of 1.8 µm over a 4 mm imaging range, with an A-scan rate of up to 300 kHz. Imaging performance is demonstrated by beam profiling and imaging esophagus ex vivo.
SignificanceEndoscopic optical coherence tomography (OCT) enables real-time optical biopsy of human organs. Endoscopic probes require miniaturization of optics, which in turn limits field of view. When larger imaging areas are needed such as in the gastrointestinal tract, the operator must manually scan the probe over the tissue to extend the field of view, often resulting in an imperfect scanning pattern and increased risk of missing lesions. Automatic scanning has the potential to extend the field of view of OCT, allowing the user to focus on image interpretation during real-time observations.AimThis work proposes an automatic scanning using a steerable OCT catheter integrated with a robotized interventional flexible endoscope. The aim is to extend the field of view of a low-profile OCT probe while improving scanning accuracy and maintaining a stable endoscope’s position during minimally invasive treatment of colorectal lesions.ApproachA geometrical model of the steerable OCT catheter was developed for estimating the volume of the accessible workspace. Experimental validation was done using electromagnetic tracking of the catheter’s positions. An exemplary scanning path was then selected within the available workspace to evaluate motion performance with the robotized steerable OCT catheter. Automatic scanning is compared to a teleoperated one and a manual scanning with a nonrobotized flexible endoscope. Spectral arc length, scanning area, spacing between scan trajectories, and time are metrics used to quantify performance.ResultsThe available scanning workspace was experimentally estimated to be 255 cm3. The automatic scanning mode provided the highest accuracy and smoothness of motion with spectral arc length of −3.18, covered area of 10.11 cm2, 1.54 mm spacing between 15 sweep trajectories, maximum translation of 27.99 mm, and time to finish of 3.11s.ConclusionsAutomatic modality improved the accuracy of scanning within a large workspace. The robotic capability provided better control to the user to define spacing resolution of scanning patterns.
Optical Coherence Tomography (OCT) shows the ability of real-time diagnosis for the external environment or internal small lumen. OCT has not been applied to larger internal lumen like the colon or stomach due to the difficulty of scanning. In this work, we use a robotized helical scanning probe to explore large lumen. Based on the segmentation of the stabilized OCT image, high accurate distance and quantitative contact feedback are obtained for the robotic scanning. The proposed method for distance/contact feedback shows robustness on both phantom and real deformable colon tissue. The robotic scanning is conducted on the soft phantom.
Side-viewing catheter-based medical imaging modalities are used to produce cross-sectional images underneath tissue surfaces. Mainstream side-viewing catheters are based on Optical Coherence Tomography (OCT) or Ultrasound, and they are often applied to the luminal environment. Automatic lumen segmentation provides geometry information for tasks like robotic control and lumen assessment for real-time diagnosis task with side-viewing catheters. In this work, we propose a novel lumen segmentation deep neural networks based on explicit coordinates encoding, which is named CE-net. CE-net is computationally efficient and produces and produces clean segmentation by explicitly encoding the boundaries coordinates in one shot. The experimental evaluation shows a processing time of approximately 8ms per frame while maintaining robustness. We propose a data generation method to improve CE-net generalization, which shows considerable performance by just training with a small dataset.
High-resolution, extended depth-of-field (EDOF) optical coherence tomography (OCT) in tethered capsules can be achieved using a mirror-tunnel design, and then further modeled and optimized for enhanced performance. In this work, a model with initial dimensions yielded 13 µm full-width-at-half-maximum (FWHM) spot size over 1.5 mm DOF. An optimization algorithm produced a design that attained 8 µm FWHM over 1.5 mm DOF. The initial and optimized probes were fabricated, beam-profiled and integrated into capsules connected to a swept-source OCT system, for imaging swine esophagus in experiments. The resulting data comparison and analysis show the lateral resolution improvement achievable in optimized probes.
The rotational distortion of endoscopic Optical Coherence Tomography (OCT) is caused by friction of optical fiber and motor instabilities. On-line rotational distortion compensation is essential to provide real-time feedback. We proposed a new method that integrates a Convolutional Neural Network based warping parameters prediction algorithm to correct the azimuthal position of each image line. This method solves the problem of drift in iterative processing by an overall shifting parameter predicting nets with a processing time of 145ms/frame and variation reduction of 88.9% for the data obtained in ex-vivo and in-vivo experiments.
Endoscopic submucosal dissection (ESD) is a minimally invasive treatment for early stage colorectal cancer that can be performed in teleoperation with a robotized flexible interventional endoscope. However, the tissue elevation step which requires submucosal needle insertion still requires manual operation. In this work we present robotic needle placement using image-guidance that combines white-light camera images to control the alignment of the needle and the OCT catheter. OCT images are used to determine the position of the needle tip during its insertion. This procedure is experimentally tested in an optical phantom that simulates the tissue layers of the colon.
Tissues-mimicking phantoms are widely used for performance evaluation of imaging systems. Disease specific design of the phantom is necessary for the correct assessment of a system’s parameters. Such phantoms are a key requirement for the continued development of various imaging techniques such as optical coherence tomography (OCT), which has been successfully applied for diagnosis of diseases in the esophagus and preliminary data show that it can be also highly perspective for diagnosis of colorectal cancer. However, in vivo validation of this novel optical approach is often difficult, since the disease model development in large animals, such as pigs, is a quite challenging task. The optimal colorectal cancer phantoms should have the following criteria: 1) realized geometry in three dimensions, 2) customizable material and optical properties, 3) mounting system allowing placement in various locations of the bench-top colon model (plastic or tissue) and removal using standard endoscopic tools, 4) visual appearance compatible with white light endoscopic imaging, and 5) long term stability. To match all these criteria, we propose tissue-mimicking phantoms prepared using 3D printing and PDMS/TiO2 insertions for cancer-like regions that are covered with the layer of Dragon skin to color-match the mucosa appearance, as we believe these materials are the most promising for durable and accurate replication of tissue properties. The polyps are mounted in the colon using small neodymium magnet embedded in the base of the polyp. The developed polyps were evaluated
using optical coherence tomography system.
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