We present a laser speckle contrast imaging (LSCI) device equipped with an image conduit to image microvascular blood flow in remote tissues like ear, nose, throat (ENT) and cervical region. The system is validated using a tissue mimicking microfluidic flow phantom with different widths and flow speeds. The proposed system is being developed as a point of care testing (POCT) device best suited for at-home self-monitoring in resource-limited areas as it is non-invasive, portable, affordable and real time.
We introduce a novel cerebral perfusion phantom to calibrate laser speckle imaging systems for small animal brain imaging. A method based on Hele-Shaw cell technique is used to create fractal like structures which serves as the mould for the phantom making. The structure is casted to create micro channels in PDMS phantoms which is found to closely mimic the structure of superficial cerebral blood vessels in small animals. The proposed method has the potential to fabricate optically and anatomically accurate cerebral perfusion phantom using a quick and inexpensive fabrication technique suitable for blood flow imaging studies.
A portable, compact and modular small-animal imaging platform is designed for measurement of deep tissue and superficial cerebral blood flow. The platform is integrated with optics, data acquisition unit, display unit and on-board single-board computer (SBC) that supports a Graphical User Interface (GUI). It also contains a customizable stereotaxic frame for both mice and rats for housing the animals along with provision for anesthesia, pulse oximeters and temperature probes. Functional studies have been conducted in the olfactory bulb and somatosensory cortex in mice brain using the imaging platform to measure relative cerebral blood flow (rCBF). We show longitudinal blood flow changes in the pre-cortical brain region associated with multiple odours, categorized broadly into ester, phenyl propanoid and terpenoids chemical group. A forepaw stimulation study has also been conducted to show blood flow changes in the cortical region of the brain. The surface as well as depth-wise blood flow changes due to the stimulations have been shown using Laser Speckle Contrast Imaging (LSCI) and Multi-speckle Diffuse Correlation Tomography (M-DCT) respectively.
We present a computationally fast algorithm for estimating the optical property distribution of turbid media using diffuse optics principles without the inversion of Jacobian matrix. The algorithm is validated by simulations and experimental studies.
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