Mr. Shengxuan Chen Profile

Shengxuan Chen

at Washington Univ in St Louis

SPIE Involvement:

Author

Publications (3)

SPIE Journal Paper | 28 November 2024 Open Access

Zika virus encephalitis causes transient reduction of functional cortical connectivity

Shannon Agner, Lindsey Brier, Jeremy Hill, Ethan Liu, Annie Bice, Rachel Rahn, Shengxuan Chen, Joseph Culver, Robyn Klein

NPh, Vol. 12, Issue S1, S14603, (November 2024) https://doi.org/10.1117/12.10.1117/1.NPh.12.S1.S14603

Read Abstract +

SignificanceDetermining the long-term cognitive impact of infections is clinically challenging. Using functional cortical connectivity, we demonstrate that interhemispheric cortical connectivity is decreased in individuals with acute Zika virus (ZIKV) encephalitis. This correlates with decreased presynaptic terminals in the somatosensory cortex. During recovery from ZIKV infection, presynaptic terminals recover, which is associated with recovered interhemispheric connectivity. This supports the contribution of synapses in the cortex to functional networks in the brain, which can be detected by widefield optical imaging. Although myeloid cell and astrocyte numbers are still increased during recovery, RNA transcription of multiple proinflammatory cytokines that increase during acute infection decreases to levels comparable to mock-infected mice during recovery. These findings also suggest that the immune response and cytokine-mediated neuroinflammation play significant roles in the integrity of brain networks during and after viral encephalitis.AimWe hypothesized that widefield optical imaging would allow us to assess functional cortical network disruption by ZIKV, including hippocampal-cortical networks.ApproachWe use widefield optical imaging to measure cortical functional connectivity (FC) in mice during acute infection with, and recovery from, intracranial infection with a mouse-adapted strain of ZIKV.ResultsAcute ZIKV infection leads to high levels of myeloid cell activation, with loss of neurons and presynaptic termini in the cerebral cortex and associated loss of FC primarily within the somatosensory cortex. During recovery, neuron numbers, synapses, and FC recover to levels near those of healthy mice. However, hippocampal injury and impaired spatial cognition persist. The magnitude of activated myeloid cells during acute infection predicted both recovery of synapses and the degree of FC recovery after recovery from ZIKV infection.ConclusionsThese findings suggest that a robust inflammatory response may contribute to the health of functional brain networks after recovery from infection.

DOWNLOAD PAPER

Proceedings Article | 28 April 2022 Poster + Presentation

Simultaneous functional and thermal imaging of mouse cortex

Hunter Banks, Shengxuan Chen, Jonathan Bumstead, Lindsey Brier, Annie Bice, Seana Gaines, Joseph Culver

Proceedings Volume PC11946, PC119460W (2022) https://doi.org/10.1117/12.2608942

KEYWORDS: Thermography, Signal to noise ratio, Brain, Neuroimaging, Infrared radiation, Infrared imaging, Two photon imaging, Two photon excitation microscopy, Optical design, Laser safety

Read Abstract +

Proceedings Article | 5 March 2021 Presentation + Paper

Automated craniotomy with impedance-sensitive skull curvature profiling

Michelle Hedlund, Hunter Banks, Annie Bice, Shengxuan Chen, Joseph Culver

Proceedings Volume 11629, 116292B (2021) https://doi.org/10.1117/12.2577682

KEYWORDS: Skull, Ultrasonography, Switches, Surgery, Spatial resolution, Scattering, Neuroimaging, In vivo imaging, Environmental sensing, Circuit switching

Read Abstract +

View contact details

UPDATE YOUR PROFILE

Is this your profile? Update it now.

Sign into your SPIE.org account

Don’t have a profile and want one?

Create an account on SPIE.org

Keywords/Phrases

Search In:

Publication Years