We introduce an innovative photoacoustic tomography approach for volumetric imaging that addresses the limitations of traditional piezoelectric ultrasound transducers. By using non-contact optical detection via digital holography, coupled with a flexible PDMS cover layer, our method maps surface displacements induced by photoacoustic waves on rough biological tissues with sub-nanometer precision. We also develop a two-step backpropagation algorithm that compensates for wave component attenuation and adjusts acoustic velocity. Our method successfully records vascular images of a mouse's hind leg and a chicken embryo's chorioallantoic membrane with a lateral resolution of 140 μm.
We developed an OCT with a wavelength of 1.7-μm swept source which has deeper penetration depth and high absorption in lipids. The OCT system imaged the brain tumor model and the optical attenuation coefficients were obtained for normal and tumor regions.
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