Dr. Thomas Dertinger Profile

Dr. Thomas Dertinger

at Univ of California Los Angeles

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Publications (7)

Proceedings Article | 21 February 2008 Paper

Latest applications for 2-focus fluorescence correlation spectroscopy

T. Dertinger, I. von der Hocht, A. Loman, R. Erdmann, J. Enderlein

Proceedings Volume 6862, 686202 (2008) https://doi.org/10.1117/12.762365

KEYWORDS: Fluorescence correlation spectroscopy, Diffusion, Refractive index, Calcium, Molecules, Luminescence, Prisms, Glasses, Proteins, Pulsed laser operation

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Proceedings Article | 14 February 2007 Paper

Monitoring of small conformational changes by high-precision measurements of hydrodynamic radius with 2-focus fluorescence correlation spectroscopy (2fFCS)

Thomas Dertinger, Iris von der Hocht, Ingo Gregor, Konstantin Komolov, Karl-Wilhelm Koch, Jörg Enderlein

Proceedings Volume 6444, 64440H (2007) https://doi.org/10.1117/12.704930

KEYWORDS: Calcium, Fluorescence correlation spectroscopy, Proteins, Luminescence, Pulsed laser operation, Prisms, Beam splitters, Polarization, Molecules, Diffusion

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Proceedings Article | 27 February 2006 Paper

Dynamic saturation optical microscopy

Jan Sýkora, Thomas Dertinger, Jörg Enderlein

Proceedings Volume 6092, 60920E (2006) https://doi.org/10.1117/12.647987

KEYWORDS: Luminescence, Microscopy, Diffraction, Modulation, Optical microscopy, Spatial resolution, Microscopes, Molecules, Sensors, Point spread functions

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Proceedings Article | 27 February 2006 Paper

Measuring precise diffusion coefficients with two-focus fluorescence correlation spectroscopy

Thomas Dertinger, Ingo Gregor, Iris von der Hocht, Rainer Erdmann, Benedikt Krämer, Felix Koberling, Rudolf Hartmann, Jörg Enderlein

Proceedings Volume 6092, 609203 (2006) https://doi.org/10.1117/12.651053

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Luminescence, Molecules, Pulsed laser operation, Polarization, Beam splitters, Objectives, Photodetectors, Data modeling

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Proceedings Article | 29 March 2005 Paper

Advanced multifocus confocal laser scanning microscope for single molecule studies

Thomas Dertinger, Felix Koberling, Ales Benda, Rainer Erdmann, Martin Hof, Joerg Enderlein

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.611147

KEYWORDS: Molecules, Luminescence, Confocal microscopy, Diffusion, Fluorescence correlation spectroscopy, Pulsed laser operation, Particles, Microscopes, Fluorescence spectroscopy, Photodetectors

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Proceedings Article | 29 March 2005 Paper

Art and artifacts of fluorescence correlation spectroscopy

Joerg Enderlein, Ingo Gregor, Digambara Patra, Thomas Dertinger

Proceedings Volume 5699, (2005) https://doi.org/10.1117/12.588527

KEYWORDS: Diffusion, Fluorescence correlation spectroscopy, Molecules, Refractive index, Confocal microscopy, Wavefronts, Monochromatic aberrations, Objectives, Sensors, Molecular interactions

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Proceedings Article | 18 June 2004 Paper

Compact TCSPC upgrade package for laser scanning microscopes based on 375- to 470-nm picosecond diode lasers

Uwe Ortmann, T. Dertinger, Michael Wahl, Matthias Patting, Rainer Erdmann

Proceedings Volume 5325, (2004) https://doi.org/10.1117/12.536888

KEYWORDS: Laser scanners, Luminescence, Sensors, Semiconductor lasers, Picosecond phenomena, Data acquisition, Microscopes, Photon counting, Signal detection, Fluorescence lifetime imaging

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We present the technical integration of state-of-the-art picosecond diode laser sources and data acquisition electronics in conventional laser scanning microscopes. This offers users of laser scanning microscopes an easy upgrade path towards time-resolved measurements. Our setup uses picosecond diode lasers from 375 nm, 405 nm, 440 nm and 470 nm for fluorescence excitation which are coupled in through a sole single mode fiber. The detected signal is guided to a photon counting detector, such as Photomultiplier Tubes (PMT) or Single Photon Avalanche Diodes (SPAD). This combines the outstanding sensitivity of photon counting detectors with the ease of use of diode laser sources, to allow time-resolved measurements of fluorescence decays with resolutions down to picoseconds. The synchronization signals from the laser scanning microscope are fed into the data stream recorded by the TimeHarp 200 TCSPC5,7 system, via the unique Time-Tagged Time-Resolved (TTTR)6 data acquisition mode. In this TTTR data acquisition mode each photon is recorded individually with its specific parameters as detector channel, picosecond timing, global arrival time and, in this special application, up to three additional markers. These markers, in combination with the global arrival time, allow the system software to reconstruct the complete image and subsequently fit the full fluorescence lifetime image. The multi-parameter data acquisition scheme of the TimeHarp 200 electronics not only records each parameter individually, but offers in addition the opportunity to analyse the parameter dependencies in a multitude of different ways. This method allows not only to calculate the fluorescence fluctuation correlation function (FCS) on any single spot of interest but also to reconstruct the fluorescence decay of each image pixel and detector channel for the purpose of Fluorescence Lifetime Imaging (FLIM) or advanced Fluorescence Resonance Energy Transfer (FRET) analysis. We present here some selected results acquired with standard laser scanning microscopes upgraded for the time-correlated single photon counting technique.

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