This study aims to apply a dual-channel SLO for in-vivo imaging of human retinal ganglion cell (RGC) transplantation in mice. Without adaptive optics, all three vascular layers were distinctly separated by vascular fluorescent labeling, and single capillaries were clearly resolved. RGCs were derived from human embryonic cells, express RGC specific tdTomato. After 9 and 14 days of RGC transplantation in C57BL/6J wild-type and Lama1-nmf223 mice, cell survival was robust and imaged by in-vivo SLO. Most RGC cells were found gathering near optic nerve head and grew extensive and lengthy neurites. Thus, our system can serve as a non-invasive tool for in-vivo monitoring of RGC repopulation.
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