Rapid 3D imaging of meso- or macro-scale organs and organisms at sub-micron resolution is challenging in life sciences. We present a static light-sheet microscopy that can achieve imaging sequences at a high speed of up to 100 fps. Our method utilizes a double-ring light modulation module in the illumination optics path to produce a low sidelobe Bessel light-sheet, providing hardware benefits for high-throughput imaging. In our study of the tumor microenvironment in human lymphoma tissue, we achieved triple-color achromatic imaging and used a custom algorithm to analyze cell co-localization, cell distance, and other relevant statistical parameters. Moreover, by combining our light-sheet system with a raster, we achieved uniform high resolution across the entire sample. Our method enables various cellular analyses at the systems level and has the potential to assist scientists in solving scientific problems.
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