To visualize neuronal structure and function in the physiological context, optical microscopy that is non-invasive and capable of resolving sub-cellular structures has become the method of choice. Structured illumination microscopy (SIM) is a widefield fluorescence imaging technique that optically sections 3D samples, but its applications have been usually limited to in vitro samples. To apply SIM to in vivo imaging, we modified optical-sectioning SIM reconstruction algorithm and incorporated adaptive optics. We demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural and functional interrogations of the brain in vivo.
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