This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

In this talk, we provide an overview of the basic principle, research fields, and representative applications of TIE, focusing particularly on optical imaging, metrology, and microscopy. In particular, we present a new type of label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography (TIDT). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements based on off-the-shelf bright-field microscope hardware, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. These results highlight a new era in which strict coherence and interferometry are no longer prerequisites for quantitative phase imaging and diffraction tomography, paving the way toward a new generation of label-free three-dimensional microscopy, with applications in all branches of biomedicine.

We present a new label-free three-dimensional (3D) microscopy technique, termed transport of intensity diffraction tomography with non-interferometric synthetic aperture (TIDT-NSA). Without resorting to interferometric detection, TIDT-NSA retrieves the 3D refractive index (RI) distribution of biological specimens from 3D intensity-only measurements at various illumination angles, allowing incoherent-diffraction-limited quantitative 3D phase-contrast imaging. The unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of complex multi-layer biological samples. Based on an off-the-shelf bright-field microscope with a programmable lightemitting-diode (LED) illumination source, we demonstrate the achievable imaging resolution of TIDT-NSA at 206 nm laterally and 0.52 um axially with a high-NA oil immersion objective and validate the 3D R

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

Structured Illumination Microscopy(SIM) is a suitable instrument for fluorescence imaging, especially dynamic imaging of live cells. It has two significant advantages of fast imaging speed and low excitation light energy density, and can reach the resolution of 100nm. In this article, we focus on the traditional reconstruction algorithm of SIM, which separates the spectrum with 9 raw images of 3-step phase-shifted picture in 3 orientations and operates the reconstruction with the correct displacement in Fourier space. However, without considering the initial phase error and the possibility that the displacement is a sub-pixel, there are artifacts in the results of the traditional reconstruction algorithm of SIM. To eliminate the artifacts and improve the imaging quality, we analyze the causes of various artifacts, and study the cross-correlation-based reconstruction algorithm of SIM, using the maximum cross-correlation value between the spectrum to get the correct displacement. Then, we simulate the illumination experimental parameters on the images of the reconstructed results. From the perspective of both hardware and software, we respectively consider the construction of the home-built SIM setup and the reconstruction software design, and finally realize the tri-color SIM system.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.

This conference presentation was prepared for the Advanced Optical Imaging Technologies V conference at Photonics Asia, 2022.