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White-light imaging remains indispensable as the standard of care, from ophthalmoscopy to endoscopy, yet the ability to capture multispectral imaging (MSI) data without changing the instrument form factor would dramatically enhance our visualization of tissue biochemistry. We introduce a custom MSI sensor that combines standard white-light with narrowband imaging and enables quantitative assessment of changes in tissue blood supply. The MSI sensor is realized by patterning a custom filter array atop a CMOS image sensor using a single-step grayscale-to-color lithographic process. By augmenting the ubiquitous Bayer mosaic with complementary narrowband filters, biomedical MSI is achieved using a single image sensor.
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Calum Williams, Jonathan Griffiths, George S. D. Gordon, Travis W. Sawyer, Sarah E. Bohndiek, "A biomedical multispectral image sensor," Proc. SPIE PC11943, Molecular-Guided Surgery: Molecules, Devices, and Applications VIII, PC1194303 (7 March 2022); https://doi.org/10.1117/12.2609373