Effects on dynamic-microscopic OCT signals after manipulation of keratinocyte metabolism in an in-vitro skin model by temperature and with metabolic reagents

Felix Hilge; Hinnerk Schulz-Hildebrandt; Michael Wang-Evers; Nunciada Salma; Martin Ahrens; Michael Münter; Gereon Hüttmann; Reginald Birngruber; Dieter Manstein

doi:10.1117/12.2612879

7 March 2022 Effects on dynamic-microscopic OCT signals after manipulation of keratinocyte metabolism in an in-vitro skin model by temperature and with metabolic reagents

Felix Hilge, Hinnerk Schulz-Hildebrandt, Michael Wang-Evers, Nunciada Salma, Martin Ahrens, Michael Münter, Gereon Hüttmann, Reginald Birngruber, Dieter Manstein

Author Affiliations +

Proceedings Volume PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI; PC1194814 (2022) https://doi.org/10.1117/12.2612879
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Microscopic optical coherence tomography (OCT) provides three-dimensional, high-resolution imaging but lacks (sub-) cellular contrast. Dynamic-microscopic OCT (dmOCT) is an approach exploiting dynamic changes of the scattering behavior in metabolically active cells. However, the underlying cellular processes responsible for those intensity fluctuations and hence the dynamic signals are not finally identified yet. Here, we present the effects of different temperatures and metabolic reagents on dmOCT images of an in-vitro human skin model. Our data indicates a dependency of the dmOCT signals on metabolic activity rather than Brownian motion and suggests dependency on the metabolic state.

Conference Presentation

Citation Download Citation

Felix Hilge, Hinnerk Schulz-Hildebrandt, Michael Wang-Evers, Nunciada Salma, Martin Ahrens, Michael Münter, Gereon Hüttmann, Reginald Birngruber, and Dieter Manstein "Effects on dynamic-microscopic OCT signals after manipulation of keratinocyte metabolism in an in-vitro skin model by temperature and with metabolic reagents", Proc. SPIE PC11948, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVI, PC1194814 (7 March 2022); https://doi.org/10.1117/12.2612879

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical coherence tomography

In vitro testing

Skin

Mode conditioning cables

Temperature metrology

3D modeling

Acquisition tracking and pointing

Show All Keywords

Keywords/Phrases

Search In:

Publication Years