Dynamic full-field optical coherence tomography of retinal pigment epithelium cell cultures to model degenerative diseases

Kassandra Groux; Jules Scholler; Anna Verschueren; Marie Darche; Leyna Boucherit; Pedro Mecê; Valérie Fradot; Jean-Marie Chassot; Mathias Fink; Sacha Reichman; Michel Paques; Claude Boccara; Olivier Thouvenin; Kate Grieve

doi:10.1117/12.2581048

5 March 2021 Dynamic full-field optical coherence tomography of retinal pigment epithelium cell cultures to model degenerative diseases

Kassandra Groux, Jules Scholler, Anna Verschueren, Marie Darche, Leyna Boucherit, Pedro Mecê, Valérie Fradot, Jean-Marie Chassot, Mathias Fink, Sacha Reichman, Michel Paques, Claude Boccara, Olivier Thouvenin, Kate Grieve

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Proceedings Volume 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV; 1163014 (2021) https://doi.org/10.1117/12.2581048
Event: SPIE BiOS, 2021, Online Only

Abstract

Dynamic FFOCT allows us to record the intrinsic motion of biological samples in 3D, over hours. We performed scratch assays on primary porcine RPE and human induced pluripotent stem cells derived RPE cell cultures. We plotted motion maps from the optical flow. For wounds <40µm, the cell layer close the wound at different speeds depending on the type of RPE cells. For bigger wounds, the cell layer retract, mimicking degenerative diseases. Comparison between Dynamic FFOCT images and Immuno-chemistry images showed that mitochondria may contribute to the dynamic profile of cells. Dynamic FFOCT can be useful for the study of regenerative medicine.

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Kassandra Groux, Jules Scholler, Anna Verschueren, Marie Darche, Leyna Boucherit, Pedro Mecê, Valérie Fradot, Jean-Marie Chassot, Mathias Fink, Sacha Reichman, Michel Paques, Claude Boccara, Olivier Thouvenin, and Kate Grieve "Dynamic full-field optical coherence tomography of retinal pigment epithelium cell cultures to model degenerative diseases", Proc. SPIE 11630, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXV, 1163014 (5 March 2021); https://doi.org/10.1117/12.2581048

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