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Calcium imaging has been widely employed in neuroscience research. A major challenge of calcium imaging is that the optical access depth in the mammalian brain is limited. Miniature optical imaging probes have been employed to image the deep brain regions. However, their imaging field-of-view is very small, which limits the number of neurons accessible and the overall experiment success rate. Recently, we have developed the Clear Optically Matched Panoramic Access Channel Technique (COMPACT), which effectively converts deep brain imaging into endoscopic microscopy. We will present the latest work of 0.5 mm COMPACT for in vivo functional imaging of mouse brain.
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