Multimodality molecular imaging and extracellular vesicle release based genetic profiling with porphyrin nanodroplets (Conference Presentation)

Roger J. Zemp; Robert J. Paproski

doi:10.1117/12.2253254

24 April 2017 Multimodality molecular imaging and extracellular vesicle release based genetic profiling with porphyrin nanodroplets (Conference Presentation)

Roger J. Zemp, Robert J. Paproski

Author Affiliations +

Proceedings Volume 10064, Photons Plus Ultrasound: Imaging and Sensing 2017; 100642F (2017) https://doi.org/10.1117/12.2253254
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

For emerging tissue-engineering applications, transplants, and cell-based therapies it is important to assess cell viability and function in vivo in deep tissues. Bioluminescence and fluorescence methods are poorly suited to deep monitoring applications with high resolution and require genetically-engineered reporters which are not always feasible. We report on a method for imaging cell viability using deep, high-resolution photoacoustic imaging. We use an exogenous dye, Resazurin, itself weakly fluorescent until it is reduced from blue to a pink color with bright red fluorescence. Upon cell death fluorescence is lost and an absorption shift is observed. The irreversible reaction of resazurin to resorufin is proportional to aerobic respiration. We detect colorimetric absorption shifts using multispectral photoacoustic imaging and quantify the fraction of viable cells. SKOV-3 cells with and without ±80oC heat treatment were imaged after Resazurin treatment. High 575nm:620nm ratiometric absorption and photoacoustic signals in viable cells were observed with a much lower ratio in low-viability populations.

Conference Presentation

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Roger J. Zemp and Robert J. Paproski "Multimodality molecular imaging and extracellular vesicle release based genetic profiling with porphyrin nanodroplets (Conference Presentation)", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 100642F (24 April 2017); https://doi.org/10.1117/12.2253254

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KEYWORDS

Absorption

Luminescence

Genetics

Molecular imaging

Nanodroplets

Photoacoustic imaging

Profiling

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