Three-dimensional reconstruction and visualization of confocal microscopic images formed in backscattered light

Barry R. Masters

doi:10.1117/12.2283767

5 August 1993 Three-dimensional reconstruction and visualization of confocal microscopic images formed in backscattered light

Barry R. Masters

Author Affiliations +

Proceedings Volume 10311, Medical Optical Tomography: Functional Imaging and Monitoring; 103110M (1993) https://doi.org/10.1117/12.2283767
Event: Medical Optical Tomography: Functional Imaging and Monitoring, 1993, Bellingham, WA, United States

Abstract

How can we use visible light to obtain a three-dimensional image of thick, living biological tissues such as the eye or skin? Confocal light microscopy can be used to obtain a stack of optical sections which are stored as digital images. These optical sections can then be processed in a digital computer to form three-dimensional reconstructions. Four-dimensional microscopy is defined as the three-dimensional reconstruction of specimens which are changing with time. At specific time intervals a set of optical sections are collected through the use of a confocal microscope. Each set of optical sections are then transformed into a three-dimensional reconstruction which corresponds to the individual stack of optical sections taken at a given time.

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Barry R. Masters "Three-dimensional reconstruction and visualization of confocal microscopic images formed in backscattered light", Proc. SPIE 10311, Medical Optical Tomography: Functional Imaging and Monitoring, 103110M (5 August 1993); https://doi.org/10.1117/12.2283767

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