A multimodal imaging platform with integrated simultaneous photoacoustic microscopy, optical coherence tomography, optical Doppler tomography and fluorescence microscopy

Arash Dadkhah; Jun Zhou; Nusrat Yeasmin; Shuliang Jiao

doi:10.1117/12.2289211

19 February 2018 A multimodal imaging platform with integrated simultaneous photoacoustic microscopy, optical coherence tomography, optical Doppler tomography and fluorescence microscopy

Arash Dadkhah, Jun Zhou, Nusrat Yeasmin, Shuliang Jiao

Author Affiliations +

Proceedings Volume 10494, Photons Plus Ultrasound: Imaging and Sensing 2018; 104940Z (2018) https://doi.org/10.1117/12.2289211
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Various optical imaging modalities with different optical contrast mechanisms have been developed over the past years. Although most of these imaging techniques are being used in many biomedical applications and researches, integration of these techniques will allow researchers to reach the full potential of these technologies. Nevertheless, combining different imaging techniques is always challenging due to the difference in optical and hardware requirements for different imaging systems. Here, we developed a multimodal optical imaging system with the capability of providing comprehensive structural, functional and molecular information of living tissue in micrometer scale. This imaging system integrates photoacoustic microscopy (PAM), optical coherence tomography (OCT), optical Doppler tomography (ODT) and fluorescence microscopy in one platform. Optical-resolution PAM (OR-PAM) provides absorption-based imaging of biological tissues. Spectral domain OCT is able to provide structural information based on the scattering property of biological sample with no need for exogenous contrast agents. In addition, ODT is a functional extension of OCT with the capability of measurement and visualization of blood flow based on the Doppler effect. Fluorescence microscopy allows to reveal molecular information of biological tissue using autofluoresce or exogenous fluorophores. In-vivo as well as ex-vivo imaging studies demonstrated the capability of our multimodal imaging system to provide comprehensive microscopic information on biological tissues. Integrating all the aforementioned imaging modalities for simultaneous multimodal imaging has promising potential for preclinical research and clinical practice in the near future.

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Arash Dadkhah, Jun Zhou, Nusrat Yeasmin, and Shuliang Jiao "A multimodal imaging platform with integrated simultaneous photoacoustic microscopy, optical coherence tomography, optical Doppler tomography and fluorescence microscopy", Proc. SPIE 10494, Photons Plus Ultrasound: Imaging and Sensing 2018, 104940Z (19 February 2018); https://doi.org/10.1117/12.2289211

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KEYWORDS

Optical coherence tomography

Imaging systems

Luminescence

Tissue optics

Multimodal imaging

Blood circulation

Tissues

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