Simultaneous confocal fluorescence microscopy and optical coherence tomography for drug distribution and tissue integrity assessment

Matthew T. Rinehart; Jeffrey LaCroix; Marcus Henderson; David Katz; Adam Wax

doi:10.1117/12.874986

21 February 2011 Simultaneous confocal fluorescence microscopy and optical coherence tomography for drug distribution and tissue integrity assessment

Matthew T. Rinehart, Jeffrey LaCroix, Marcus Henderson, David Katz, Adam Wax

Author Affiliations +

Proceedings Volume 7890, Advanced Biomedical and Clinical Diagnostic Systems IX; 789010 (2011) https://doi.org/10.1117/12.874986
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

The effectiveness of microbicidal gels, topical products developed to prevent infection by sexually transmitted diseases including HIV/AIDS, is governed by extent of gel coverage, pharmacokinetics of active pharmaceutical ingredients (APIs), and integrity of vaginal epithelium. While biopsies provide localized information about drug delivery and tissue structure, in vivo measurements are preferable in providing objective data on API and gel coating distribution as well as tissue integrity. We are developing a system combining confocal fluorescence microscopy with optical coherence tomography (OCT) to simultaneously measure local concentrations and diffusion coefficients of APIs during transport from microbicidal gels into tissue, while assessing tissue integrity. The confocal module acquires 2-D images of fluorescent APIs multiple times per second allowing analysis of lateral diffusion kinetics. The custom Fourier domain OCT module has a maximum a-scan rate of 54 kHz and provides depth-resolved tissue integrity information coregistered with the confocal fluorescence measurements. The combined system is validated by imaging phantoms with a surrogate fluorophore. Time-resolved API concentration measured at fixed depths is analyzed for diffusion kinetics. This multimodal system will eventually be implemented in vivo for objective evaluation of microbicide product performance.

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Matthew T. Rinehart, Jeffrey LaCroix, Marcus Henderson, David Katz, and Adam Wax "Simultaneous confocal fluorescence microscopy and optical coherence tomography for drug distribution and tissue integrity assessment", Proc. SPIE 7890, Advanced Biomedical and Clinical Diagnostic Systems IX, 789010 (21 February 2011); https://doi.org/10.1117/12.874986

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