Digital field-of-view correction for dual-view transport-of-intensity equation-based quantitative microscopy

Mingyuan Zhang; Qingtao Gong; Qi Wei; Jing Xu; Yan Kong; Zhilong Jiang; Cheng Liu; Shouyu Wang

doi:10.1117/12.2500585

23 October 2018 Digital field-of-view correction for dual-view transport-of-intensity equation-based quantitative microscopy

Mingyuan Zhang, Qingtao Gong, Qi Wei, Jing Xu, Yan Kong, Zhilong Jiang, Cheng Liu, Shouyu Wang

Author Affiliations +

Proceedings Volume 10820, Optics in Health Care and Biomedical Optics VIII; 108201M (2018) https://doi.org/10.1117/12.2500585
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

As an ideal way for quantitative live cell imaging, dual view transport of intensity equation (TIE) method can provide both real time imaging, multi-mode observations, simple setup and large field of view (FoV). However, the image recorder installation error reduces the accuracy in both amplitude and phase retrievals, because of the inevitable FoV mismatch between the captured under- and over-focus intensities. In order to obtain higher accuracy amplitude and phase retrievals, the phase correlation based digital FoV correction is introduced into our method, rotation, scale and translation between the under- and over-focus images are compensated by the phase correlation based digital FoV correction. Measurements are implemented using standard sample detection and quantitative live cell imaging, proving that the proposed method can improve the accurate of the amplitude and phase computations.

Citation Download Citation

Mingyuan Zhang, Qingtao Gong, Qi Wei, Jing Xu, Yan Kong, Zhilong Jiang, Cheng Liu, and Shouyu Wang "Digital field-of-view correction for dual-view transport-of-intensity equation-based quantitative microscopy ", Proc. SPIE 10820, Optics in Health Care and Biomedical Optics VIII, 108201M (23 October 2018); https://doi.org/10.1117/12.2500585

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