Real-time cellular phase imaging using dual-view transport of intensity phase microscopy

Shaowei Sun; Zhilong Jiang; Yan Kong; Xiaoliang He; Cheng Liu; Shouyu Wang

doi:10.1117/12.2548834

20 December 2019 Real-time cellular phase imaging using dual-view transport of intensity phase microscopy

Shaowei Sun, Zhilong Jiang, Yan Kong, Xiaoliang He, Cheng Liu, Shouyu Wang

Proceedings Volume 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019); 112093F (2019) https://doi.org/10.1117/12.2548834
Event: Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 2019, Xi'an, China

Abstract

Transport of intensity phase microscopy (TIPM) can obtain sample phase images with commercial microscope and simple procedures; however, it requires multi-focal images limiting its real-time detection capability. In order to achieve dynamics of the live cells, we adopted dual-view TIPM which simultaneously captures under- and over-focus images for phase retrieval. In dual-view TIPM, two identify CMOS cameras were first set on the binocular tube of the microscope with different focal position of 3 mm difference to simultaneously capture both the under- and over-focus images. Afterwards, scaling, rotation and shifting are all corrected to maintain the same fields of view of under- and over-focus images. Next, the cellular phase can be extracted by solving the Poisson equation. The dual-view TIPM was finally adopted in live cell imaging, proving the dual-view TIPM can obtain real-time quantitative cellular phase imaging.

Citation Download Citation

Shaowei Sun, Zhilong Jiang, Yan Kong, Xiaoliang He, Cheng Liu, and Shouyu Wang "Real-time cellular phase imaging using dual-view transport of intensity phase microscopy", Proc. SPIE 11209, Eleventh International Conference on Information Optics and Photonics (CIOP 2019), 112093F (20 December 2019); https://doi.org/10.1117/12.2548834

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