Evaluating the potential for resistance development in Gram-negative bacteria to antimicrobial blue light (at 405 nm): in vitro and in vivo studies

Leon G. Leanse; Olivia D. Harrington; Yanyan Fang; Imran Ahmed; Xueping Sharon Goh; Tianhong Dai

doi:10.1117/12.2506910

7 March 2019 Evaluating the potential for resistance development in Gram-negative bacteria to antimicrobial blue light (at 405 nm): in vitro and in vivo studies

Leon G. Leanse, Olivia D. Harrington, Yanyan Fang, Imran Ahmed, Xueping Sharon Goh, Tianhong Dai

Author Affiliations +

Proceedings Volume 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II; 1086318 (2019) https://doi.org/10.1117/12.2506910
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

With the increasing number of pathogenic microbes that are becoming resistant to routinely used antimicrobials, it is important to look to non-traditional approaches for the treatment of infections. Antimicrobial blue light (aBL;405 nm) is a novel strategy for the treatment of infections. Here we report an investigation into the potential for resistance development to aBL in three clinically important Gram-negative bacteria, through sequential exposure in vitro and in vivo. We found that 20 cycles of aBL exposure, in vitro, did not incur resistance development, in any of the species tested (Acinetobacter baumanii, Pseudomonas aeruginosaor Escherichia coli). In addition, sub-curative sequential aBL treatment of a wound infected with a bioluminescent variant of the P. aeruginosa PAO1 strain, did not influence sensitivity to aBL. In conclusion, it is unlikely that sequential treatment of aBL will result in resistance generation, suggesting that multiple treatments of aBL may be administered without resistance development becoming a concern.

Citation Download Citation

Leon G. Leanse, Olivia D. Harrington, Yanyan Fang, Imran Ahmed, Xueping Sharon Goh, and Tianhong Dai "Evaluating the potential for resistance development in Gram-negative bacteria to antimicrobial blue light (at 405 nm): in vitro and in vivo studies", Proc. SPIE 10863, Photonic Diagnosis and Treatment of Infections and Inflammatory Diseases II, 1086318 (7 March 2019); https://doi.org/10.1117/12.2506910

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