Single blood cell Raman spectroscopy reveals elevated haemoglobin content in poikilocytosis

Suet Man Tsui; Rafay Ahmed; Condon Lau

doi:10.1117/12.2507697

4 March 2019 Single blood cell Raman spectroscopy reveals elevated haemoglobin content in poikilocytosis

Suet Man Tsui, Rafay Ahmed, Condon Lau

Proceedings Volume 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII; 108811A (2019) https://doi.org/10.1117/12.2507697
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Abnormally shaped red blood cells (poikilocytes) can cause serious health problems such as anemia and increase the risk of death. At present, the biochemical abnormalities in poikilocytes is not well understood, especially at the single cell level. In this study, confocal Raman spectroscopy revealed biochemical differences between single normal red blood cells (RBCs) and poikilocytes. Intragastric administration of nanoparticulate titanium dioxide (TiO₂) was used to produce poikilocytes. Adult rats were administered by gavage 200mg/kg body weight TiO₂ every other day for 20 days (low-dose, N=5) or 250mg/kg every day for 60 days (high-dose, N=5). Low and high-dose controls (N=5 each) were administered distilled water for equal durations. Raman spectroscopy was performed on individual RBCs of low-dose subjects using 514nm excitation and a confocal setup. Whole blood from high-dose subjects underwent a Complete Blood Count (CBC) and inductively coupled plasma mass spectrometry (ICP-MS). Acanthocytes and echinocytes, two types of poikilocytes, were observed from TiO₂ subjects. RBCs were grouped into four types: normal RBCs from controls and normal-looking RBCs, Acanthocytes, and Echinocytes from TiO₂ subjects. The intensities of Raman bands at 1637, 1585, 1559, 1372 and 1228cm^-1 are larger in acanthocytes than normal and normal-looking RBCs. The 1342cm^-1 band is larger in normal RBCs, acanthocytes and echinocytes than in normal-looking RBCs. Also, the 975cm^-1 band is larger in acanthocytes than normal-looking RBCs. These bands are associated with oxygenated RBCs. Overall, poikilocytes, especially acanthocytes, carry more oxygen and haemoglobin and this is corroborated by CBC and ICPMS.

Conference Presentation

Citation Download Citation

Suet Man Tsui, Rafay Ahmed, and Condon Lau "Single blood cell Raman spectroscopy reveals elevated haemoglobin content in poikilocytosis", Proc. SPIE 10881, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, 108811A (4 March 2019); https://doi.org/10.1117/12.2507697

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