Phase transition monitoring in adipose tissue by multiphoton microscope

Irina Yu. Yanina; Viktor V. Nikolaev; Vyacheslav I. Kochubey; Valery V. Tuchin; Yury V. Kistenev

doi:10.1117/12.2526927

22 July 2019 Phase transition monitoring in adipose tissue by multiphoton microscope

Irina Yu. Yanina, Viktor V. Nikolaev, Vyacheslav I. Kochubey, Valery V. Tuchin, Yury V. Kistenev

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110761O (2019) https://doi.org/10.1117/12.2526927
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

In the present study, observations of temperature-mediated phase transitions of the components of adipose tissue were done. Pieces of pork abdominal fat were used as samples of adipose tissue. These samples were heated using a temperature-controlled table and observed using a MPTflex two-photon microscope (JenLab GmbH) with fluorescence lifetime imaging microscopy (FLIM) mode. The analysis of FLIM data was done with a software package “Becker&Hickl”. The developed approach has a great potential for obtaining accurate information on the phase-transition processes occurring during metabolism alterations.

Citation Download Citation

Irina Yu. Yanina, Viktor V. Nikolaev, Vyacheslav I. Kochubey, Valery V. Tuchin, and Yury V. Kistenev "Phase transition monitoring in adipose tissue by multiphoton microscope", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761O (22 July 2019); https://doi.org/10.1117/12.2526927

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