Imaging dental sections with polarization-resolved SHG and time-resolved autofluorescence

Jun Huang Chen; Po-Yen Lin; Stephen C. Y. Hsu; Fu-Jen Kao

doi:10.1117/12.808524

13 February 2009 Imaging dental sections with polarization-resolved SHG and time-resolved autofluorescence

Jun Huang Chen, Po-Yen Lin, Stephen C. Y. Hsu, Fu-Jen Kao

Proceedings Volume 7183, Multiphoton Microscopy in the Biomedical Sciences IX; 71831N (2009) https://doi.org/10.1117/12.808524
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

In this study, we are using two-photon (2-p) excited autofluorescence and second harmonic (SH) as imaging modalities to investigate dental sections that contains the enamel and the dentin. The use of near-infrared wavelengths for multiphoton excitation greatly facilitates the observation of these sections due to the hard tissue's larger index of refraction and highly scattering nature. Clear imaging can be achieved without feature altering preparation procedures of the samples. Specifically, we perform polarization resolving on SH and lifetime analysis on autofluorescence. Polarization resolved SH reflects the preferred orientation of collagen while very different autofluorescence lifetimes are observed from the dentin and the enamel. The origin of 2-p autofluorescence and SH signals are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils exist only in the dentin and dentinoenamel junctions.

Citation Download Citation

Jun Huang Chen, Po-Yen Lin, Stephen C. Y. Hsu, and Fu-Jen Kao "Imaging dental sections with polarization-resolved SHG and time-resolved autofluorescence", Proc. SPIE 7183, Multiphoton Microscopy in the Biomedical Sciences IX, 71831N (13 February 2009); https://doi.org/10.1117/12.808524

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available